![]() We find that TOE1 promotes maturation of all regular RNA polymerase II-transcribed snRNAs of the major and minor spliceosomes by removing post-transcriptional oligo(A)-tails, trimming 3’ ends, and preventing Ify a central role for the human DEDD deadenylase TOE1 in distingushing the fates of small nuclear (sn)RNAs of the spliceosome from genome-encoded snRNA variants. Ĭompetition between 3’ end maturation and degradation drives snRNA quality controlīioproject:PRJNA594409 scription: Accordingly, TOE1 deficiency impaired telomerase activity. Using siRNA and CRISPR-Cas9 knockout technology, we found that deficiency of TOE1 resulted in accumulation of polyadenylated TERC precursors, while did not decrease the levels of total TERC. TOE1 immunoprecipitation complex harbors telomerase activity in a DEDD dependent way. We identified a protein TOE1 accumulated specifically in Cajal bodies, where telomerase RNP assemblies and TERC accumulates. Homo sapiens : Biallelic mutations in the 3’ exonuclease TOE1 cause Pontocerebellar Hypoplasia Type 7 and result in snRNA processing defectsīiallelic mutations in the 3’ exonuclease TOE1 cause Pontocerebellar Hypoplasia Type 7 and result in snRNA processing defects PARN and TOE1 constitute a 3′ end maturation module for nuclear non-coding RNAs Removed Target of EGR1 protein 1 C3H1-type Nuclear localization signal N-acetylala. Cell cycle defects were also often observed. ![]() Screening the overexpression array revealed that 64 transcription factor genes exhibited reduced fitness when ectopically expressed. ![]() Here, we systematically overexpressed 99 transcription factor genes with the nmt1 promoter. Omplicating their functional characterization and target gene identification. ![]() RNA polymerase II-transcribed spliceosomal small nuclear RNAs (snRNAs) initiates with cleavage by the Integrator complex, but the factors that subsequently remove 3’ end extensions from metaz.įunctional Characterization of Fission Yeast Transcription Factors by Overexpression Analysis Solution structure of the zf-CCCH domain of target of EGR1, member 1 ( Nuclear)īiallelic loss of human TOE1 links snRNA processing to pontocerebellar neurodegeneration ![]()
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